Antimicrobial Activity of Glucoprotamin: A Clinical Study of a New Disinfectant for Instruments

摘要

Abstract Objective: To determine the in vitro efficacy of glucoprotamin for the disinfection of instruments. Design: Prospective observational study. Setting: University women's hospital. Methods: Instruments were immersed in saline solution after use, and glucoprotamin was added to a concentration of 1.5% before soaking for 60 minutes. Biocidal activity was determined by the difference in colony-forming units (CFU) on instruments before and after disinfection. Results: One hundred thirty-seven instruments were collected during 10 days and exposed to a 1.5% dilution of glucoprotamin without prior washing. Bioburden before disinfection ranged from 2 × 105 to 7.1 × 107 CFU per instrument. Average bacterial killing was 5.98 log10 CFU ± 0.48 under aerobic conditions and 6.75 log10 CFU ± 0.54 under anaerobic conditions, despite the presence of large amounts of proteins on instruments that were frequently bloody. No vegetative bacteria were isolated in any sample after disinfection. Conclusion: This clinical study confirmed excellent in vitro efficacy of glucoprotamin without prior removal of proteins and debris